The SRY and SOX proteins share a DNA-binding domain known as the HMG box, defined by a 79-amino
acid region. All SOX proteins have a single HMG box and bind linear DNA in a sequence-specific manner, resulting in
the bending of DNA through large angles. Bending causes the DNA helix to open for some distance, which may affect
binding and interactions of other transcription factors.
Nucleic acid binding, DNA binding, Transcription factor
Kanda H, et al 1998 reported that rainbow trout Sox24, a novel member of the Sox family, is a transcriptional regulator during oogenesis.
The authors isolated a cDNA clone encoding a novel SRY-type HMG box (Sox) protein,
designated Sox24, from a rainbow trout ovary cDNA library. On the basis of the
HMG box amino acid sequence, Sox24 can be categorized into the same subgroup
of Sox proteins as SOX4, SOX11, and SOX22. The proteins in this group also
share a highly conserved sequence at the C-terminus. The Sox24 mRNA is
expressed at high levels in the ovary, and in-situ hybridization localized its
expression to oocytes. The recombinant protein containing the Sox24 HMG box
region bound to an AACAAT sequence strongly in a gel retardation assay. Upon
co-transfection into CHO cells, the full-length Sox24 transactivated transcription
from a reporter plasmid through the AACAAT binding motif.