Stanford Home
Ovarian Kaleidoscope Database (OKdb)

Home

History

Transgenic Mouse Models

INFORGRAPHICS

Search
Submit
Update
Chroms
Browse
Admin

Hsueh lab

HPMR

Visits
since 01/2001:
176557

decorin OKDB#: 1694
 Symbols: DCN Species: human
 Synonyms: CSCD, PG40, PGII, PGS2, DSPG2, SLRR1B  Locus: 12q21.33 in Homo sapiens


For retrieval of Nucleotide and Amino Acid sequences please go to: OMIM Entrez Gene
Mammalian Reproductive Genetics   Endometrium Database Resource   Orthologous Genes   UCSC Genome Browser   GEO Profiles new!   Amazonia (transcriptome data) new!

R-L INTERACTIONS   MGI

DNA Microarrays
SHOW DATA ...
link to BioGPS
General Comment Large chondroitin sulfate proteoglycans were first identified in hyaline cartilage, where they specifically interact with hyaluronan and form large supramolecular complexes. Together with other matrix glycoproteins, they provide mechanical support and a fixed negative charge. The core protein of fibroblast chondroitin sulfate proteoglycan was designated versican in recognition of its versatile modular structure. Decorin (125255) and biglycan (301870) are 2 other soft tissue proteoglycans. Decorin and biglycan (301870) are related but distinct small proteoglycans found in many connective tissues.

NCBI Summary: This gene encodes a member of the small leucine-rich proteoglycan family of proteins. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature protein. This protein plays a role in collagen fibril assembly. Binding of this protein to multiple cell surface receptors mediates its role in tumor suppression, including a stimulatory effect on autophagy and inflammation and an inhibitory effect on angiogenesis and tumorigenesis. This gene and the related gene biglycan are thought to be the result of a gene duplication. Mutations in this gene are associated with congenital stromal corneal dystrophy in human patients. [provided by RefSeq, Nov 2015]
General function Ligand, Extracellular binding protein
Comment
Cellular localization Extracellular Matrix, Secreted
Comment
Ovarian function Luteinization
Comment Decorin is a part of the ovarian extracellular matrix in primates and may act as a signaling molecule. Adam M et al. STUDY QUESTIONIs decorin (DCN), a putative modulator of growth factor (GF) signaling, expressed in the primate ovary and does it play a role in ovarian biology?SUMMARY ANSWERDCN expression in the theca, the corpus luteum (CL), its presence in the follicular fluid (FF) and its actions revealed in human IVF-derived granulosa cells (GCs), suggest that it plays multiple roles in the ovary including folliculogenesis, ovulation and survival of the CL.WHAT IS KNOWN ALREADYDCN is a secreted proteoglycan, which has a structural role in the extracellular matrix (ECM) and also interferes with the signaling of multiple GF/GF receptors (GFRs). However, DCN expression and action in the primate ovary has yet to be determined.STUDY DESIGN, SIZE, DURATIONArchival human and monkey ovarian samples were analyzed. Studies were conducted using FF and GC samples collected from IVF patients.PARTICIPANTS/MATERIALS, SETTING, METHODSImmunohistochemistry, western blotting, RT-PCR, quantitative RT-PCR (qPCR) and enzyme-linked immunosorbent assay (ELISA) studies were complemented by cellular studies, including the measurements of intracellular Ca(2+), reactive oxygen species (ROS), epidermal GF receptor (EGFR) phosphorylation by DCN and caspase activity.MAIN RESULTS AND THE ROLE OF CHANCEImmunohistochemistry revealed strong DCN staining in the connective tissue and follicular thecal compartments, but not in GCs of pre-antral and antral follicles. Pre-ovulatory follicles could not be studied, but DCN was associated with connective tissue of CL samples and the cytoplasm of luteal cells. DCN expression in monkey CL doubled (P < 0.05) towards the end of the luteal lifespan. DCN was found in human FF obtained from IVF patients (mean: 12.9 ng/ml; n = 20) as determined by ELISA. DCN mRNA and/or protein were detected in freshly isolated and cultured, luteinized human GCs. In the latter, exogenous human recombinant DCN increased intracellular Ca(2+) levels and induced the production of ROS in a concentration-dependent manner. DCN, like epidermal GF, phosphorylated EGFR significantly (P < 0.05) and reduced the activity of caspase 3/7 in cultured GCs. The data indicate the expression of DCN in the theca of growing follicles, in FF of ovulatory follicles and in the CL. Therefore, DCN may exert paracrine actions via GF/GFR systems in multiple ovarian compartments.LIMITATIONS, REASONS FOR CAUTIONFunctional studies were performed in cultures of human luteinized GCs, which are an apt model but may not fully mirror the pre-ovulatory GC compartment or the CL. Other human ovarian cells, including the thecal cells, were not available.WIDER IMPLICATIONS OF THE FINDINGSIn accordance with its evolving roles in other organs, ovarian DCN is an ECM-associated component, which acts as a multifunctional regulator of GF signaling in the primate ovary. DCN may thus be involved in folliculogenesis, ovulation and the regulation of the CL survival in primates.STUDY FUNDING/COMPETING INTEREST(S)This study was supported by Deutsche Forschungsgemeinschaft (DFG) MA1080/17-3 and in part DFG MA1080/21-1 (to AM), NIH grants HD24870 (S.R.O. and R.L.S.), the Eunice Kennedy Shriver NICHD/NIH through cooperative agreement HD18185 as part of the Specialized Cooperative Centers Program in Reproduction and Infertility Research (S.R.O.) and 8P51OD011092-53 for the operation of the Oregon National Primate Research Center (G.A.D., J.D.H., S.R.O. and R.L.S).
Expression regulated by LH
Comment
Ovarian localization Oocyte, Granulosa, Theca, Luteal cells
Comment Molecular cloning, expression analysis, and function of decorin in goat ovarian granulosa cells. Peng JY et al. (2016) Decorin (DCN), a component of the extracellular matrix (ECM), participates in ECM assembly and influences cell proliferation and apoptosis in many mammalian tissues and cells. However, expression and function of DCN in the ovary remain unclear. This study cloned the full-length cDNA of goat DCN obtained from the ovary of an adult goat. Sequence analysis revealed that the putative DCN protein shared a highly conserved amino acid sequence with known mammalian homologs. The tissue distribution of DCN mRNA expression was evaluated by real-time PCR, and the results showed that DCN was widely expressed in the tissues of adult goat. Immunohistochemistry results suggested that DCN protein existed in the granulosa cells and oocytes from all types of follicles and theca cells of antral follicles. Moreover, hCG-induced DCN mRNA expression was significantly reduced by the inhibitors of protein kinase A, PI3K, or p38 kinase (P < 0.05), which are key mediators involved in hCG-induced DCN expression. Overexpression of DCN significantly increased apoptosis and blocked cell cycle progression in cultured granulosa cells (P < 0.05). Western blot analysis also showed that overexpression of DCN upregulated the expression levels of p21 protein (P < 0.05), whereas no effects were observed on the expression of Bax and Bcl-2 and on Bcl-2/Bax ratio (P > 0.05). These findings suggested that DCN regulates the apoptosis and cell cycle of granulosa cells.////////////////// Large chondroitin sulfate proteoglycans were first identified in hyaline cartilage, where they specifically interact with hyaluronan and form large supramolecular complexes. Together with other matrix glycoproteins, they provide mechanical support and a fixed negative charge. The core protein of fibroblast chondroitin sulfate proteoglycan was designated versican in recognition of its versatile modular structure. Decorin (125255) and biglycan (301870) are 2 other soft tissue proteoglycans.
Follicle stages Antral, Preovulatory
Comment
Phenotypes
Mutations 0 mutations
Genomic Region show genomic region
Phenotypes and GWAS show phenotypes and GWAS
Reprogenomic viewer show phenotypes and GWAshow RNAseq data and genomic region in Reprogenomic viewer site
(After opening the Reprogenomics Viewer site, select your gene and click on Chromosome + Link)
Links
OMIM (Online Mendelian Inheritance in Man: an excellent source of general gene description and genetic information.)
OMIM \ Animal Model
KEGG Pathways
Recent Publications
None
Search for Antibody
Discuss..
blog comments powered by Disqus
Related Genes
Beta
Show data ...


created: Jan. 29, 2003, 9:18 a.m. by: hsueh   email:
home page:
last update: Aug. 30, 2016, 10:38 a.m. by: hsueh    email:



Use the back button of your browser to return to the Gene List.

Click here to return to gene search form