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Hsueh lab

HPMR

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176557

Roundabout, Drosophila, Homolog Of, 3 OKDB#: 3800
 Symbols: ROBO3 Species: human
 Synonyms: HGPS, RIG1, HGPPS, RBIG1, FLJ21044,RB-INHIBITING GENE 1, RBIG1, RIG1  Locus: 11q24.2 in Homo sapiens
HPMR


For retrieval of Nucleotide and Amino Acid sequences please go to: OMIM Entrez Gene
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General Comment
General function Receptor
Comment
Cellular localization Plasma membrane
Comment
Ovarian function Luteolysis
Comment
Expression regulated by
Comment
Ovarian localization Luteal cells
Comment Novel regulated expression of the SLIT/ROBO pathway in the ovary: possible role during luteolysis in women. Dickinson RE et al. The human corpus luteum (CL) undergoes luteolysis, associated with marked tissue and vascular remodeling, unless conception occurs and the gland is 'rescued' by human chorionic gonadotropin (hCG). In Drosophila the Slit gene product, a secreted glycoprotein, acts as a ligand for the roundabout (robo) transmembrane receptor. Together they influence the guidance and migration of neuronal and non-neuronal cells. In vertebrates three Slit (Slit1, Slit2, Slit3) and four Robo (Robo1, Robo2, Robo3/Rig-1, Robo4/Magic Robo) genes have been identified. ROBO1, SLIT2 and SLIT3 are also inactivated in human cancers and may regulate apoptosis and metastasis. Since processes such as apoptosis and tissue remodeling occur during the regression of the CL, the aim of this study was to investigate the expression, regulation and effects of the SLIT and ROBO genes in human luteal cells. Immunohistochemistry and RT-PCR revealed that SLIT2, SLIT3, ROBO1 and ROBO2 are expressed in luteal steroidogenic cells and fibroblast-like cells of the human CL. Furthermore, using real-time quantitative PCR, expression of SLIT2, SLIT3, and ROBO2 was maximal in the late-luteal phase and significantly reduced after luteal 'rescue' in vivo with exogenous hCG (P<0.05). Additionally, hCG significantly inhibited SLIT2, SLIT3 and ROBO2 expression in cultured luteinized granulosa cells (P<0.05). Blocking SLIT-ROBO activity increased migration and significantly decreased levels of apoptosis in primary cultures of luteal cells (P<0.05). Overall these results suggest the SLIT/ROBO pathway could play an important role in luteolysis in women.
Follicle stages Corpus luteum
Comment
Phenotypes
Mutations 0 mutations
SNPs
D E M O
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created: June 25, 2008, 10:09 a.m. by: hsueh   email:
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last update: June 25, 2008, 10:09 a.m. by: hsueh    email:



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